The plant tissue is first excised from the selected part of the mother plant. Depending on the age and the strain of the mother plant, the excision regions vary and the key is to obtain the most responsive part of plant tissue. The excised tissue is then carefully sterilized to remove bacterial contamination that will impede plant tissue growth in the later part of the micropropagation process. The sterilization process has to be precise to destroy all the bacteria and bacterial spores but not to the extent of causing damage or mutation to the plant tissue.
The sterilized plant tissue is then exposed to specific chemicals to revert the cells from their differentiated stages. The treated plant tissue is then incubated under controlled conditions. During the incubation stages, a percentage of treated plant tissue will not survive to the subsequent stages due to development failure. The surviving plant tissue that is responsive will be selected and multiplied by a few orders of magnitude.
By the end of the micropropagation process, batches of plant tissue are regenerated into plantlet during the maturation stage. The matured plantlets are ready for field plantation.
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